{"id":28287,"date":"2022-08-22T12:00:00","date_gmt":"2022-08-22T12:00:00","guid":{"rendered":"https:\/\/www.cancer.gov\/news-events\/cancer-currents-blog\/2022\/pancreatic-cancer-collagen-treatment-target"},"modified":"2024-11-03T15:13:17","modified_gmt":"2024-11-03T15:13:17","slug":"abnormal-collagen-may-be-weak-spot-for-pancreatic-cancer","status":"publish","type":"post","link":"https:\/\/ogkologos.com\/en\/abnormal-collagen-may-be-weak-spot-for-pancreatic-cancer\/","title":{"rendered":"Abnormal Collagen May Be Weak Spot for Pancreatic Cancer"},"content":{"rendered":"

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Collagen is made of fibrous protein that provides structural support to many tissues in the body.<\/p>\n

Credit: Tom Deerinck and Mark Ellisman, NICMR<\/p>\n<\/p><\/div>\n<\/figcaption><\/figure>\n<\/p><\/div>\n

A protein called collagen, which provides structure to tissues, is found almost everywhere in the human body, from the skin to the bones. Pancreatic cancer cells can also produce their own misshapen collagen<\/a>, a new study has found.<\/p>\n

And this abnormal collagen appears to have additional cancer-promoting functions. In experiments in mice, shutting down the production or effects of the abnormal collagen made treatments for pancreatic cancer more effective<\/a>.<\/p>\n

In the NCI-supported study, the research team found that the abnormal collagen ramped up activity in pancreatic cancer cells that increased tumor growth and survival.<\/p>\n

But when the research team stopped cancer cells in mice from producing the abnormal collagen, everything changed. Perhaps most importantly, cancer-fighting immune cells<\/a> started to move into the tumors. And when the researchers then treated the mice with a commonly used immunotherapy<\/a> drug, their tumors shrank dramatically. The immunotherapy drugs were minimally effective in mice that had the abnormal collagen.<\/p>\n

The study findings were published August 8 in Cancer Cell<\/em> by Raghu Kalluri, M.D., Ph.D., of the University of Texas MD Anderson Cancer Center, and his colleagues.<\/p>\n

\u201cWhen the collagen is present around the cancer cells, it\u2019s functioning like a cloaking device so that the immune cells aren\u2019t able to recognize those cancer cells, or get to those cancer cells. So [they are] almost invisible to the immune system. But then when this collagen is removed, the immune cells are able to see and kill them,” Dr. Kalluri said.<\/p>\n

This abnormal collagen makes a potentially promising therapeutic target, he explained, because it\u2019s only produced by cancer cells. <\/p>\n

\u201cThis is what everyone wants to find: an abnormal protein that\u2019s specific to cancer cells,\u201d said Grace Ault, Ph.D., of NCI\u2019s Division of Cancer Biology<\/a>, who was not part of the study.<\/p>\n

Any potential strategies to target this abnormal collagen are years away from clinical testing, Dr. Ault warned. But these early results suggest that such an approach may offer an important advantage.<\/p>\n

Different collagens, different functions<\/h2>\n

Pancreatic cancer is notoriously resistant to commonly used treatments. Among other roadblocks, pancreatic tumors become packed with stromal (supportive) cells and the molecules they produce, including collagen. \u201cSometimes, the [cancer] cells are actually a minority of the cells in a pancreatic tumor,\u201d explained Dr. Ault. <\/p>\n

Normal tissues rely on collagen to perform many of their functions. A protein with a branched, fiber-like structure made up of chains called \u03b11 and \u03b12, it gives tissues both strength and flexibility.<\/p>\n

\u201cIt\u2019s the most abundant protein in the human body. It\u2019s present in our bones, ligaments, and cartilage\u2014it\u2019s everywhere,\u201d said Dr. Kalluri.<\/p>\n

Collagen is normally produced by cells called fibroblasts<\/a>. Past work from Dr. Kalluri and his team using mice found that the presence of normal collagen may actually help suppress pancreatic cancer growth.<\/p>\n

But other research had suggested that pancreatic cancer cells can produce their own collagen. Whether this collagen is different\u2014and whether it helps tumors survive and grow\u2014wasn\u2019t well understood.<\/p>\n

When Dr. Kalluri and his colleagues reviewed a database that captures the molecular makeup of human cancer cell lines<\/a> used for research, they noticed that the collagen produced by pancreatic cancer cells had a different structure. Rather than two \u03b11 chains and one \u03b12 chain, called a heterotrimer, it only contained \u03b11 chains, producing an abnormal structure called a homotrimer. <\/p>\n

This abnormal collagen didn\u2019t appear to be the result of any gene changes (mutations<\/a>) in the cancer cells. Instead, its production was driven by a difference in how the genes responsible for collagen production were expressed, through a process called methylation<\/a>. This action, known as an epigenetic change, turned off the gene that normally produces the \u03b12 chain.<\/p>\n

When they looked at mice engineered to develop pancreatic tumors, they found that the abnormal collagen appeared to be promoting rather than suppressing tumor growth.<\/p>\n

When the researchers engineered mice to develop pancreatic cancer that couldn\u2019t produce the abnormal collagen, tumors grew more slowly, and the mice lived longer. Their tumors also had more of the tumor-suppressing collagen normally produced by fibroblasts.<\/p>\n

Cancer cells isolated from the mice lacking the abnormal collagen and grown in the lab were slower to divide and more sensitive to the chemotherapy drug gemcitabine (Gemzar)<\/a>, a standard treatment for pancreatic cancer, than cancer cells that could still produce the abnormal collagen. The cells lacking the abnormal collagen were also slower to form tumors when implanted into other mice.<\/p>\n

Uncloaking a tumor<\/h2>\n

How does the abnormal collagen promote tumor growth? Further work in pancreatic cancer cells isolated from mice found that the homotrimers were somehow boosting a communication pathway that can drive runaway cell growth. In contrast, normal collagen suppressed this pathway.<\/p>\n

The researchers then zeroed in on a receptor<\/a> on the surface of pancreatic cancer cells called \u03b13\u03b21 integrin<\/a>, which seemed to be turning on this signaling pathway<\/a> when the abnormal collagen bound to it. When the researchers shut down this receptor in pancreatic cancer cells, the cells stopped growing and dividing, even those that still produced the abnormal collagen. <\/p>\n

When the research team looked for \u03b13\u03b21 integrin in tumor samples taken from 130 people with pancreatic cancer, they found that almost all expressed high amounts of the receptor.<\/p>\n

Tumor samples with the highest levels of the receptor had fewer immune cells called T cells<\/a> than tumors that had proportionally lower levels of the receptor. And people with tumors containing the highest levels of the receptor died sooner. <\/p>\n

Looping back to mice, several good things appeared to happen in pancreatic tumors engineered to lack the abnormal collagen. Among other things, it changed the microbiome<\/a> of the tumor: the microorganisms and viruses that live in the tumor tissue.<\/p>\n

The fact that tumors deep in the body can have resident microbes is a relatively recent discovery in cancer biology, explained Dr. Ault.<\/p>\n

\u201cIt used to be thought that bacteria were mostly found in places [in the body] that were ‘open,’ like the mouth or the colon,\u201d said Dr. Ault. \u201cAnd people thought that inside, in contained tissues [like the pancreas] you didn’t have all these bacteria. But it turns out they’re all over.\u201d<\/p>\n

In tumors in mice that were engineered to lack the abnormal collagen, the populations of bacteria that lived in the tumors shifted compared with those found in nonengineered mice. These microbial changes were associated with the presence of more immune cells, such as T cells, in the tumor.<\/p>\n

But the bacteria weren\u2019t the only things linked to these immune cell changes in the tumors. Shutting down the aberrant collagen, they showed, also blocked signaling by the cancer cells that directly impeded immune cells, allowing T cells to flood into the now-friendlier tumor microenvironment<\/a>.<\/p>\n

As a result, mice with tumors that couldn\u2019t produce the abnormal collagen lived longer when treated with an immune checkpoint inhibitor<\/a>, a type of immunotherapy, than mice with tumors that could produce the homotrimer. <\/p>\n

Harnessing a potential vulnerability<\/h2>\n

\u201cMaking this homotrimer [may be] a very early event in a cancer cell’s journey,\u201d said Dr. Kalluri. \u201cBut more work needs to be done to connect the dots [as to how this happens],\u201d he added.<\/p>\n

Mutations in a cancer-causing gene called KRAS<\/em>, which are present in almost all pancreatic tumors, may be causing the initial shutdown of normal \u03b12 collagen chain production, he explained, but further study is needed to tease out if this is the case.<\/p>\n

Regardless of the initial events that lead to a buildup of this abnormal collagen, Dr. Kalluri and his team are now looking at whether it could be a target for new cancer treatments in people.<\/p>\n

One strategy they\u2019re exploring is developing drugs that can shut down \u03b13\u03b21 integrin, which could potentially block abnormal growth signaling even in the presence of the collagen homotrimer.<\/p>\n

Combining such a strategy with other treatments has potential, explained Dr. Ault. While blocking the activity of the abnormal collagen might not kill cancer cells, it could make them more sensitive to chemotherapy or immunotherapy, she explained.<\/p>\n

The researchers would also like to engineer CAR T cells that can bind to the abnormal collagen. Since this collagen is only found in and around cancer cells, \u201cthat could direct [such T cells] to go to the cancer cells, not anywhere else,\u201d Dr. Kalluri explained.<\/p>\n

\u201cWe’d also like to understand if this [collagen] is unique to pancreatic cancer, or if it\u2019s found in other cancers as well,\u201d he added. Early results from his lab suggest that the homotrimer might also be found in lung, colon, and head and neck cancer cells, he explained, expanding the potential pool of cancer types that might be targeted with such an approach.<\/p>\n

\u201cBut to understand how widespread this [collagen] is, we still need to do more work,\u201d he said.<\/p>\n

Source<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

August 22, 2022, by NCI Staff Collagen is made of fibrous protein that provides structural support to many tissues in the body. Credit: Tom Deerinck and Mark Ellisman, NICMR A protein called collagen, which provides structure to tissues, is found almost everywhere in the human body, from the skin to the bones. Pancreatic cancer cells […]<\/p>\n","protected":false},"author":2,"featured_media":28288,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_monsterinsights_skip_tracking":false,"footnotes":""},"categories":[5137],"tags":[],"class_list":["post-28287","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-world-news"],"_links":{"self":[{"href":"https:\/\/ogkologos.com\/en\/wp-json\/wp\/v2\/posts\/28287","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/ogkologos.com\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/ogkologos.com\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/ogkologos.com\/en\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/ogkologos.com\/en\/wp-json\/wp\/v2\/comments?post=28287"}],"version-history":[{"count":1,"href":"https:\/\/ogkologos.com\/en\/wp-json\/wp\/v2\/posts\/28287\/revisions"}],"predecessor-version":[{"id":43233,"href":"https:\/\/ogkologos.com\/en\/wp-json\/wp\/v2\/posts\/28287\/revisions\/43233"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/ogkologos.com\/en\/wp-json\/wp\/v2\/media\/28288"}],"wp:attachment":[{"href":"https:\/\/ogkologos.com\/en\/wp-json\/wp\/v2\/media?parent=28287"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/ogkologos.com\/en\/wp-json\/wp\/v2\/categories?post=28287"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/ogkologos.com\/en\/wp-json\/wp\/v2\/tags?post=28287"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}